His4 pep4
WebbThe PEP4 gene encodes for the vacuolar proteinase A, which is required for the activation of several other vacuolar proteases. The PRB1 gene codes for proteinase B. SMD1163, … Webbarg4 his4 ura3 [29] JC308 ade1 arg4 his4 ura3 [29] Protease-deficient strains KM71 Δaox1::SARG4 his4 arg4 [7] MC100-3 Δaox1::SARG4Δaox2::Phis4 his4 arg4 [9] SMD1168 Δpep4::URA3 his4 ura3 [38] SMD1165 prb1 his4 [38] SMD1163 pep4 prb1 his4 [38] SMD1168 kex1::SUC2 Δpep4::URA3Δkex1::SUC2 his4 ura3 [34]
His4 pep4
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Webb1 jan. 2012 · The histidinol dehydrogenase gene ( HIS4) has been mutated to allow the selection of successful transformants based on histidine auxotrophy. The SMD1163 strain is also a protease-deficient strain lacking the active genes coding for proteinase A ( PEP4) and proteinase B ( PRB1 ). Webb28 sep. 2009 · Nevertheless, GS115 (his4) cells in flask culture secreted 3.5 mg/L of a histidine-tagged ATF-saporin chimera showing an IC 50 of 6 X 10 −11 M against U937 cells, thus demonstrating the suitability of this expression platform for secretion of toxic saporin-based chimeras.—Lombardi, A., Bursomanno, S., Lopardo, T., Traini, R., …
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Webb1 juni 2007 · The α3 isoform is abundant in nervous tissues and present at lower levels in heart and vessels and the α4 isoform is a testis-specific isoform. The β2 isoform is mainly expressed in neural tissue and β3 in testis, retina, liver and lung (Blanco & Mercer, 1998; Jorgensen, 2003 ). http://person.au.dk/fil/18925233/1998__tPA__Pg_activation__Biochem.pdf
Webb13 okt. 2024 · The resulting vector pPHT-G12 containing GFP/ HA-GSA12 behind the constitutive and glucose-inducible GAPDH promoter was linearized by cutting within the HIS4 gene (e.g., with Stu I) and used to transform by electroporation R2 ( his4 gsa12 ::Zeocin) and DMM1 ( his4 :: pDM1 (P AOX1 BFP-SKL, Zeocin)) cells.
WebbTable L Yeast Strains Strain Genotype Source JHRY20-2C JHRY20-2C vplg-A l JHRY61-1B JHRY64-5B SF838-1D SF838-1D vpl3-A1 SF838-1Dm220 SF838-1Dm 108 SF838 … m16 x 1.5 thread dimensionsWebb5 dec. 2024 · The P. pastoris SMD1168 (his4, pep4) is a protease-deficient strain that can grow in methanol at a similar rate to the wild type (Mut +). The Mut + strain requires a higher concentration of methanol inducer than the Mut s and Mut-strain. In general, methanol with a concentration of 0.5%–1.5% is used for recombinant protein expression. kiss lunch boxesWebbTargeted gene knockouts play an important role in the study of gene function. For the generation of knockouts in the industrially important yeast Pichia pastoris, several protocols have been published to date.Nevertheless, creating a targeted knockout in P. pastoris still is a time-consuming process, as the existing protocols are labour intensive … m 1700 spline twoWebbPEP4, PRB1 XM_002493288, XM_002489831 Aspartic-type endopeptidase, serine-type endopeptidase his4 pep4::URA3 prb1::URA3 Purchased from Invitrogen GS115 … kissly bra reviewsWebb30 okt. 2013 · The lacZ gene was cloned into a HIS4-based. Pichia vector and the Zeocin-resistant pPICZ. B Pichia vector. DNA was linearized at a unique. restriction site in the … m17.0 - bilateral prim osteoarthritis kneeWebbFinally, the Pep4/PrB–deficient strain is deficient in proteolytic activity of all three of these enzymes: proteinase A, carboxypeptidase Y, and proteinase B. These protease … kiss lunchbox for saleWebbConstruction of HIS4 Gene Fusions A SUC2-HIS4 gene fusion was constructed in the yeast shuttle vector YCp50, which is a low copy number plasmid that contains URA3 as … m17.11 - uni prim osteoarthritis rt knee