WebApr 6, 2024 · Then, 1 ml of GST and GST-CcSSP1 sonicated soluble supernatant was incubated with 30 μl PureCube Glutathione MagBeads (Cube Biotech) at 4°C for 1 h. The supernatant was then removed, and MagBeads were added to the supernatant expressing His-MaPR1 and incubated at 4°C for 1 h. GST fusion proteins were eluted with reduced … WebNov 17, 2024 · The right panel shows that the RH27-His protein (arrowhead) was pulled-down by GST-DCL1, GST-DDL, GST-HYL1, and GST-SE, using glutathione MagBeads, as detected by the anti-His antibody. Three independent experiments were performed.

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WebGlutathione is coupled to the magnetic agarose beads to obtain an affinity matrix with highest binding capacity for GST fusion proteins. Because the purification method … WebPureCube Glutathione Agarose and MagBeads [PDF] The GST tag is a 26 kDa protein that binds to glutathione-coupled agarose resins. This tag is considerably larger than the His tag and is often chosen to promote recombinant protein folding. Cube Biotech offers PureCube Glutathione Agarose and prepacked, FPLC-compatible columns for efficient ... the ic diet

Magbead at Thomas Scientific

WebThe GenScript Glutathione MagBeads are superparamagnetic beads, average 40 µm in diameter, coupled with reduced glutathione (GSH). The beads are supplied as 25% slurry in phosphate buffered saline (PBS), … WebAlternatively, Glutathione MagBeads are also available, if you prefer this affinity chromatography approach. Additional support products for GST-tag purification assays are L-Glutathione (reduced), an essential compound of the elution buffer, and our GST-tag antibody for later western blot detection of your protein. Web丁香通为您找到22条dna分选纯化磁珠信息，包括dna分选纯化磁珠报价行情，优质供应商，图片，品牌等最新信息，丁香通为买家提供用户服务，诚信保障等服务，批发采购dna分选纯化磁珠,上丁香通。 the ic briefing