Ad293 cell line
WebMar 12, 2014 · The nonphagocytic embryonic kidney cell line AD293 was transfected with plasmids encoding a membrane-targeted RFP (PM-RFP; A) or TIM4 fused to mCherry (TIM4-mCherry; B, C). Transfectants were exposed to phagocytic targets bearing PtdSer, biotinylated PtdEth, and PtdCho or biotinylated PtdEth and PtdCho only, to assess their … WebJun 5, 2015 · All cell lines, including INS-1 Ad293, CHO, HeLa, Pt67, SHG44, and PC12 were propagated in Dulbecco's Modified Eagle Medium (Life Technologies, Rockvile, MD) supplemented with 10% fetal calf serum (FCS, HyCLONE, Logan, UT), 2 mM glutamine, 50 U/ml of penicillin G sodium and 50 μg/ml of streptomycin sulfate (Beyotime, Haimen, …
Ad293 cell line
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WebOct 10, 2024 · Human hepatoma cell lines (HepG2 and Huh7 cells) and a mouse immortalized hepatocyte cell line (AML12) were obtained from the ATCC and cultured as previously described . SuperFect (QIAGEN, Hilden, Germany) or polyethylenimine (Polysciences, Inc., Warrington, PA, USA) were used for transient transfections … WebMay 6, 2016 · AD293 cells overexpressing wild-type EGFR, despite a significant proliferation increase in serum supplemented medium, underwent apoptosis after EGF …
WebThe calcium cell line overexpresses natural Gq-coupled wild-type GPCRs and is designed to detect calcium mobilization in response to receptor agonist stimulation. You can use the Calcium No WashPLUS detection kit to detect the signal. Native calcium GPCR detection provides a fast and reliable method for detecting changes in intracellular calcium. WebJul 21, 2024 · Here, we describe in detail the co-expression of different IpLITR-types in the human embryonic AD293 cell line and examination of their receptor cross-talk mechanisms during the regulation of the phagocytic response using imaging flow cytometry, confocal microscopy, and immunoprecipitation protocols.
WebMar 7, 2024 · Briefly, AD293 cells transfected with WT HSP90α or HSP90β were treated with either Celastrol, 17-AAG, or geldanamycin for 4 h. Cells were lysed in lysis buffer containing 50 mM Tris-HCl pH... WebAug 16, 2010 · These cell lines allow successful propagation of E1-defective Ad35 vectors to similar viral titers as Ad5. An Ad11 packaging cell line was also developed, 293-Ad11-E1B55K, which was produced by transfecting and generating a stable clone from 293 cells with a plasmid containing the Ad11 E1B55K gene under the control of human PGK .
WebJul 15, 2024 · A–C AD293, HeLa and A375 cells were treated with DMSO (control), or MG132 (2 μM), and Bafilomycin A1 (25 nM), alone or in combination for 14 h. The whole-cell lysates were subjected to western ...
WebII. Freezing the Adeno-X 293 Cell Line Once you have started growing the cell line, prepare frozen aliquots to provide a renewable source of cells. 1. Trypsinize the desired number of flasks or plates. 2. Pool cell suspensions together, count cells, and calculate total viable cell number. 3. Centrifuge cells at 100 x g for 5 min. Aspirate the ... how to add carl bot colorsWebDec 11, 2024 · Cells were maintained at 28°C, 125 rpm in a Multitron incubator (Infors HT, Annapolis Junction, MD, USA). Both HEK293T (ATCC, Manassas, VA, USA) and Ad293 cell lines (ATCC) were cultured in DMEM (Gibco, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS; Gibco). methane time in atmosphereWebThe 293 Cell Line is a permanent line established from primary embryonic human kidney transformed with human adenovirus type 5 DNA. The genes encoded by the E1 region of … how to add carfax report to autotrader adWebJul 5, 2011 · AD293 cells were treated with the indicated concentrations of CQ for 4 hours and then stained with LysoTracker Red, which labels all low-pH compartments. The rectangles represent 1.5× magnifications of regions from within the images; images were obtained by 100×/oil objective lens. Scale bars, 10 μm. methane to carbon dioxide equationWebJan 9, 2024 · The adenoviral particles were packaged and amplified in AD293 cells. At 72 h postinfection, the amplified virus was harvested by freeze-thawing three times and centrifuged at 4 °C for 15 min. ... To test if these antibodies also recognize mouse Ano5, we generated a stable cell line expressing mouse Ano5 fused with mCherry at the N … methane to carbon dioxideWebAug 16, 2010 · Apart from the HEK293 cell line which was generated with sheared Ad5 DNA, the main strategy for generating producer cell lines for E1 trans-complementation … how to add card to iphone 5WebAdeno-X™ 293 Cell Line Author: Clontech Laboratories Subject: Protocol for using Adeno-X 293 cells with the Adeno-X Expression Systems. The Adeno-X 293 Cell Line (Cat. No. … methane three dimensional structure